Detailed information of TA system TA025552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3236171..3236719
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: MCAN_RS15190
• Protein ID: WP_003414602.1
• Coordinates: 3236456..3236719 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TFG4
• Locus tag: MCAN_RS15185
• Protein ID: WP_014001470.1
• Coordinates: 3236171..3236452 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS15160	3231794..3232651	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
MCAN_RS15165	3232693..3233277	-	585	WP_003414585.1	DUF1707 domain-containing protein	-
MCAN_RS15170	3233381..3233629	+	249	WP_003913411.1	antitoxin VapB23	-
MCAN_RS15175	3233626..3234006	+	381	WP_014001468.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS15180	3234088..3235899	-	1812	WP_014001469.1	penicillin-binding protein	-
MCAN_RS15185	3236171..3236452	+	282	WP_014001470.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
MCAN_RS15190	3236456..3236719	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MCAN_RS15195	3237093..3237947	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
MCAN_RS15200	3238003..3239166	-	1164	WP_014001471.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MCAN_RS15205	3239183..3240397	-	1215	WP_014001472.1	zinc metalloprotease Rip	-
MCAN_RS15210	3240405..3241646	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T25552 WP_003414602.1 NC_015848:3236456-3236719 [Mycobacterium canettii CIPT 140010059]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T25552 NC_015848:3236456-3236719 [Mycobacterium canettii CIPT 140010059]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACGACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10210.47 Da
• Isoelectric Point: 4.4805

>AT25552 WP_014001470.1 NC_015848:3236171-3236452 [Mycobacterium canettii CIPT 140010059]
MRILPISKIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT25552 NC_015848:3236171-3236452 [Mycobacterium canettii CIPT 140010059]
ATGCGGATACTGCCGATTTCGAAGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: G0TFG4