Detailed information of TA system TA025551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3195319..3195923
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TFC8
• Locus tag: MCAN_RS14990
• Protein ID: WP_014001449.1
• Coordinates: 3195319..3195711 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFC9
• Locus tag: MCAN_RS14995
• Protein ID: WP_014001450.1
• Coordinates: 3195708..3195923 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS14970	3191774..3192562	-	789	WP_015288384.1	CRISPR-associated endoribonuclease Cas6	-
MCAN_RS14975	3192713..3193972	+	1260	WP_178125320.1	DDE-type integrase/transposase/recombinase	-
MCAN_RS14980	3194487..3195032	-	546	WP_014001448.1	DUF1802 family protein	-
MCAN_RS14985	3195029..3195298	-	270	WP_015288386.1	DUF2277 family protein	-
MCAN_RS14990	3195319..3195711	-	393	WP_014001449.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS14995	3195708..3195923	-	216	WP_014001450.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MCAN_RS15000	3195970..3196719	+	750	WP_014001451.1	enoyl-CoA hydratase	-
MCAN_RS15005	3196733..3197815	-	1083	WP_014001452.1	ABC transporter ATP-binding protein	-
MCAN_RS15010	3197808..3199118	-	1311	WP_014001453.1	ABC transporter substrate-binding protein	-
MCAN_RS15015	3199121..3199948	-	828	WP_014001454.1	carbohydrate ABC transporter permease	-
MCAN_RS15020	3199945..3200856	-	912	WP_014001455.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14697.79 Da
• Isoelectric Point: 7.0742

>T25551 WP_014001449.1 NC_015848:c3195711-3195319 [Mycobacterium canettii CIPT 140010059]
VTTVLLDSHVAYWWSAEPERLSQTASRAVEQADELAVAAISWFELAWLAEHERIHLAIPVLSWLRQLAEYLRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T25551 NC_015848:c3195711-3195319 [Mycobacterium canettii CIPT 140010059]
GTGACGACGGTGTTGCTCGACTCCCATGTGGCCTACTGGTGGTCGGCCGAGCCCGAACGTCTGAGCCAGACCGCGAGTCG
GGCCGTCGAGCAGGCCGATGAGCTTGCCGTCGCCGCGATTTCGTGGTTCGAACTGGCTTGGCTTGCCGAACATGAACGCA
TCCACCTCGCGATTCCCGTGCTGTCCTGGCTTCGGCAGCTGGCCGAGTACCTCCGCACCGTCGGCATCACTCCATCCGTC
GCCGCTACGGCGGTGGCGCTGCCCTCGTCGTTTCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACGGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGAAGTCATCGGCATCCGCGCCCGGTCACGGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7522.58 Da
• Isoelectric Point: 5.6330

>AT25551 WP_014001450.1 NC_015848:c3195923-3195708 [Mycobacterium canettii CIPT 140010059]
MAATEVKAKILALLDEVAQGEEIEITKHGRTVARLVGATGPHALKGRFRGVAMTAVDDDELFTTGVSWNAS

Nucleotide

• Length: 216 bp

>AT25551 NC_015848:c3195923-3195708 [Mycobacterium canettii CIPT 140010059]
ATGGCCGCTACGGAGGTGAAGGCGAAGATCCTCGCCTTGCTTGATGAAGTGGCCCAAGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGGAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCCGCGGTGTGGCGATGA
CGGCCGTCGACGACGACGAACTCTTCACCACCGGGGTGTCCTGGAACGCCTCGTGA

Structures

Toxin

• AlphaFold DB: G0TFC8

Antitoxin

• AlphaFold DB: G0TFC9