Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4415071..4415647 Replicon chromosome
Accession NZ_CP103557
Organism Escherichia coli O25b:H4-ST131 strain 4993

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag M5T49_RS22105 Protein ID WP_001295743.1
Coordinates 4415360..4415647 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag M5T49_RS22100 Protein ID WP_000063148.1
Coordinates 4415071..4415373 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T49_RS22085 (4411667) 4411667..4413817 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5T49_RS22090 (4413867) 4413867..4414070 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5T49_RS22095 (4414081) 4414081..4415037 + 957 WP_001295745.1 GTPase -
M5T49_RS22100 (4415071) 4415071..4415373 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
M5T49_RS22105 (4415360) 4415360..4415647 - 288 WP_001295743.1 BrnT family toxin Toxin
M5T49_RS22110 (4415844) 4415844..4417010 + 1167 WP_000800831.1 restriction endonuclease -
M5T49_RS22115 (4417074) 4417074..4418693 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
M5T49_RS22120 (4418683) 4418683..4419987 + 1305 WP_000535012.1 restriction endonuclease subunit S -
M5T49_RS22125 (4420082) 4420082..4420576 + 495 Protein_4337 type I restriction endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T255499 WP_001295743.1 NZ_CP103557:c4415647-4415360 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT255499 WP_000063148.1 NZ_CP103557:c4415373-4415071 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References