Detailed information of TA system TA255497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4036714..4038403
• Replicon: chromosome
• Accession: NZ_CP103557
• Organism: Escherichia coli O25b:H4-ST131 strain 4993

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: M5T49_RS20280
• Protein ID: WP_000365807.1
• Coordinates: 4036714..4037634 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5T49_RS20285
• Protein ID: WP_001295795.1
• Coordinates: 4037621..4038403 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5T49_RS20240 (4032187)	4032187..4032897	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
M5T49_RS20245 (4033204)	4033204..4033533	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
M5T49_RS20250 (4033545)	4033545..4033711	-	167	Protein_3973	tail fiber assembly protein	-
M5T49_RS20255 (4033782)	4033782..4034396	-	615	WP_001019923.1	YagU family protein	-
M5T49_RS20260 (4034611)	4034611..4034796	-	186	WP_000146240.1	hypothetical protein	-
M5T49_RS20265 (4034845)	4034845..4035003	+	159	Protein_3976	integrase	-
M5T49_RS20270 (4035230)	4035230..4035565	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
M5T49_RS20275 (4035773)	4035773..4036549	+	777	WP_000171155.1	site-specific integrase	-
M5T49_RS20280 (4036714)	4036714..4037634	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5T49_RS20285 (4037621)	4037621..4038403	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5T49_RS20290 (4038687)	4038687..4041785	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3996498..4045466	48968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T255497 WP_000365807.1 NZ_CP103557:c4037634-4036714 [Escherichia coli O25b:H4-ST131]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT255497 WP_001295795.1 NZ_CP103557:c4038403-4037621 [Escherichia coli O25b:H4-ST131]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0