Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3853407..3854025 Replicon chromosome
Accession NZ_CP103557
Organism Escherichia coli O25b:H4-ST131 strain 4993

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag M5T49_RS19420 Protein ID WP_001291435.1
Coordinates 3853807..3854025 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag M5T49_RS19415 Protein ID WP_000344800.1
Coordinates 3853407..3853781 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T49_RS19405 (3848496) 3848496..3849689 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M5T49_RS19410 (3849712) 3849712..3852861 + 3150 WP_032154085.1 efflux RND transporter permease AcrB -
M5T49_RS19415 (3853407) 3853407..3853781 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
M5T49_RS19420 (3853807) 3853807..3854025 + 219 WP_001291435.1 HHA domain-containing protein Toxin
M5T49_RS19425 (3854199) 3854199..3854750 + 552 WP_000102539.1 maltose O-acetyltransferase -
M5T49_RS19430 (3854866) 3854866..3855336 + 471 WP_000136192.1 YlaC family protein -
M5T49_RS19435 (3855500) 3855500..3857050 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
M5T49_RS19440 (3857092) 3857092..3857445 - 354 WP_000878135.1 DUF1428 family protein -
M5T49_RS19450 (3857824) 3857824..3858135 + 312 WP_000409908.1 MGMT family protein -
M5T49_RS19455 (3858166) 3858166..3858738 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T255496 WP_001291435.1 NZ_CP103557:3853807-3854025 [Escherichia coli O25b:H4-ST131]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT255496 WP_000344800.1 NZ_CP103557:3853407-3853781 [Escherichia coli O25b:H4-ST131]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References