Detailed information of TA system TA025549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3169632..3170202
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TFA6
• Locus tag: MCAN_RS14830
• Protein ID: WP_014001430.1
• Coordinates: 3169632..3169988 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8I0EPM8
• Locus tag: MCAN_RS14835
• Protein ID: WP_015288373.1
• Coordinates: 3169972..3170202 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS14810	3165157..3166845	-	1689	Protein_2915	alpha/beta hydrolase family protein	-
MCAN_RS14815	3166849..3167175	-	327	WP_014001427.1	hypothetical protein	-
MCAN_RS14820	3167334..3167909	+	576	WP_014001428.1	DUF3558 domain-containing protein	-
MCAN_RS14825	3167928..3169613	+	1686	WP_014001429.1	CocE/NonD family hydrolase	-
MCAN_RS14830	3169632..3169988	-	357	WP_014001430.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
MCAN_RS14835	3169972..3170202	-	231	WP_015288373.1	ribbon-helix-helix domain-containing protein	Antitoxin
MCAN_RS14840	3170245..3171288	-	1044	WP_014001431.1	DUF2293 domain-containing protein	-
MCAN_RS22505	3171302..3171457	-	156	WP_145974198.1	DUF1802 family protein	-
MCAN_RS14850	3171712..3171987	+	276	WP_014001432.1	DUF1778 domain-containing protein	-
MCAN_RS21760	3171984..3172487	+	504	WP_015288375.1	GNAT family N-acetyltransferase	-
MCAN_RS14860	3172556..3172959	+	404	Protein_2925	hypothetical protein	-
MCAN_RS14865	3172956..3173147	+	192	WP_014001435.1	hypothetical protein	-
MCAN_RS14870	3173411..3173650	-	240	WP_015288376.1	hypothetical protein	-
MCAN_RS14875	3173666..3173926	-	261	WP_015288377.1	hypothetical protein	-
MCAN_RS22510	3174196..3174464	-	269	Protein_2929	hypothetical protein	-
MCAN_RS21770	3174520..3174861	+	342	WP_080602971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12900.85 Da
• Isoelectric Point: 10.0052

>T25549 WP_014001430.1 NC_015848:c3169988-3169632 [Mycobacterium canettii CIPT 140010059]
VMRRGEIWQVDLDPARGSEANKQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAVELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T25549 NC_015848:c3169988-3169632 [Mycobacterium canettii CIPT 140010059]
GTGATGCGCCGCGGTGAGATATGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAAGCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCGACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTACCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTGCGGCCGATCGGCCGAGTTTCAGCCGTCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8259.07 Da
• Isoelectric Point: 4.1809

>AT25549 WP_015288373.1 NC_015848:c3170202-3169972 [Mycobacterium canettii CIPT 140010059]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDVWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT25549 NC_015848:c3170202-3169972 [Mycobacterium canettii CIPT 140010059]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGTGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: G0TFA6