Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 288920..289720 Replicon chromosome
Accession NZ_CP103557
Organism Escherichia coli O25b:H4-ST131 strain 4993

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag M5T49_RS01340 Protein ID WP_000342452.1
Coordinates 289193..289720 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag M5T49_RS01335 Protein ID WP_001277107.1
Coordinates 288920..289186 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T49_RS01315 (284579) 284579..285247 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
M5T49_RS01320 (285240) 285240..286298 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
M5T49_RS01325 (286543) 286543..287397 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
M5T49_RS01330 (287668) 287668..288771 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
M5T49_RS01335 (288920) 288920..289186 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
M5T49_RS01340 (289193) 289193..289720 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
M5T49_RS01345 (289717) 289717..290100 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
M5T49_RS01350 (290523) 290523..291632 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
M5T49_RS01355 (291680) 291680..292606 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
M5T49_RS01360 (292603) 292603..293880 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
M5T49_RS01365 (293877) 293877..294644 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T255482 WP_000342452.1 NZ_CP103557:289193-289720 [Escherichia coli O25b:H4-ST131]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT255482 WP_001277107.1 NZ_CP103557:288920-289186 [Escherichia coli O25b:H4-ST131]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References