Detailed information of TA system TA025548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3129813..3130500
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: MCAN_RS14615
• Protein ID: WP_003414064.1
• Coordinates: 3130105..3130500 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: MCAN_RS14610
• Protein ID: WP_003414061.1
• Coordinates: 3129813..3130079 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS14585	3125452..3126354	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MCAN_RS14590	3126423..3127175	-	753	WP_014001406.1	FAD-dependent thymidylate synthase	-
MCAN_RS14595	3127419..3127694	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
MCAN_RS14600	3127691..3129313	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
MCAN_RS14605	3129400..3129816	-	417	WP_014001407.1	type II toxin-antitoxin system toxin ribonuclease C21	-
MCAN_RS14610	3129813..3130079	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MCAN_RS14615	3130105..3130500	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS14620	3130497..3130766	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
MCAN_RS14625	3130776..3131870	-	1095	WP_014001408.1	restriction endonuclease subunit S	-
MCAN_RS14630	3131867..3132286	-	420	WP_014001409.1	hypothetical protein	-
MCAN_RS23175	3132360..3132540	-	181	Protein_2879	hypothetical protein	-
MCAN_RS14635	3132619..3133875	+	1257	WP_014001439.1	IS110 family transposase	-
MCAN_RS14640	3133887..3134366	-	480	WP_003414073.1	dihydrofolate reductase	-
MCAN_RS14645	3134437..3135237	-	801	WP_003911953.1	thymidylate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T25548 WP_003414064.1 NC_015848:c3130500-3130105 [Mycobacterium canettii CIPT 140010059]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T25548 NC_015848:c3130500-3130105 [Mycobacterium canettii CIPT 140010059]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT25548 WP_003414061.1 NC_015848:c3130079-3129813 [Mycobacterium canettii CIPT 140010059]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT25548 NC_015848:c3130079-3129813 [Mycobacterium canettii CIPT 140010059]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACCAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64