Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2082939..2083529 Replicon chromosome
Accession NZ_CP103554
Organism Klebsiella michiganensis strain 5088

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag M5R35_RS09895 Protein ID WP_014229979.1
Coordinates 2083197..2083529 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag M5R35_RS09890 Protein ID WP_004852307.1
Coordinates 2082939..2083196 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R35_RS09865 (M5R35_09865) 2078195..2078800 - 606 WP_032750440.1 glutathione S-transferase family protein -
M5R35_RS09870 (M5R35_09870) 2078980..2079906 + 927 WP_014229983.1 LysR substrate-binding domain-containing protein -
M5R35_RS09875 (M5R35_09875) 2079944..2080942 - 999 WP_014229982.1 aldo/keto reductase -
M5R35_RS09880 (M5R35_09880) 2081043..2081957 + 915 WP_014229981.1 LysR family transcriptional regulator -
M5R35_RS09885 (M5R35_09885) 2082148..2082228 - 81 Protein_1938 molybdopterin-binding protein -
M5R35_RS09890 (M5R35_09890) 2082939..2083196 + 258 WP_004852307.1 antitoxin Antitoxin
M5R35_RS09895 (M5R35_09895) 2083197..2083529 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M5R35_RS09905 (M5R35_09905) 2083852..2085309 + 1458 WP_014229916.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
M5R35_RS09915 (M5R35_09915) 2085672..2086162 + 491 Protein_1942 Arm DNA-binding domain-containing protein -
M5R35_RS09920 (M5R35_09920) 2086145..2086291 - 147 Protein_1943 DNA primase -
M5R35_RS09925 (M5R35_09925) 2086954..2088012 + 1059 WP_014229913.1 DNA cytosine methyltransferase -
M5R35_RS09930 (M5R35_09930) 2088025..2088207 + 183 WP_228343140.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2082939..2094165 11226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T255471 WP_014229979.1 NZ_CP103554:2083197-2083529 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT255471 WP_004852307.1 NZ_CP103554:2082939-2083196 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References