Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 14588..15324 Replicon plasmid pMB8806_2
Accession NZ_CP103552
Organism Klebsiella pneumoniae strain 2791

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag M5T34_RS26840 Protein ID WP_003026803.1
Coordinates 14842..15324 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag M5T34_RS26835 Protein ID WP_003026799.1
Coordinates 14588..14854 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T34_RS26815 (M5T34_26815) 10564..12264 + 1701 WP_023292074.1 AIPR family protein -
M5T34_RS26820 (M5T34_26820) 12450..13570 + 1121 WP_087759376.1 IS3 family transposase -
M5T34_RS26825 (M5T34_26825) 13662..13991 + 330 WP_023292075.1 hypothetical protein -
M5T34_RS26830 (M5T34_26830) 14187..14381 - 195 WP_023292076.1 hypothetical protein -
M5T34_RS26835 (M5T34_26835) 14588..14854 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
M5T34_RS26840 (M5T34_26840) 14842..15324 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
M5T34_RS26845 (M5T34_26845) 15536..16882 + 1347 WP_020314316.1 ISNCY family transposase -
M5T34_RS26850 (M5T34_26850) 17043..17174 + 132 WP_004218042.1 hypothetical protein -
M5T34_RS26855 (M5T34_26855) 17383..18548 - 1166 Protein_20 IS3 family transposase -
M5T34_RS26860 (M5T34_26860) 18725..19687 - 963 WP_004152113.1 zinc metalloprotease HtpX -
M5T34_RS26865 (M5T34_26865) 19674..20162 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 12450..18410 5960
- inside Non-Mobilizable plasmid aac(3)-IIa / dfrA14 - 1..55976 55976


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T255466 WP_003026803.1 NZ_CP103552:14842-15324 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT255466 WP_003026799.1 NZ_CP103552:14588-14854 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References