Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5287110..5287735 Replicon chromosome
Accession NZ_CP103550
Organism Klebsiella pneumoniae strain 2791

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M5T34_RS25730 Protein ID WP_002882817.1
Coordinates 5287110..5287493 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5T34_RS25735 Protein ID WP_004150355.1
Coordinates 5287493..5287735 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T34_RS25715 (5284476) 5284476..5285378 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5T34_RS25720 (5285375) 5285375..5286010 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M5T34_RS25725 (5286007) 5286007..5286936 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5T34_RS25730 (5287110) 5287110..5287493 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M5T34_RS25735 (5287493) 5287493..5287735 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5T34_RS25740 (5287940) 5287940..5288857 + 918 WP_009484979.1 alpha/beta hydrolase -
M5T34_RS25745 (5288871) 5288871..5289812 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5T34_RS25750 (5289857) 5289857..5290294 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5T34_RS25755 (5290291) 5290291..5291151 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5T34_RS25760 (5291145) 5291145..5291744 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T255463 WP_002882817.1 NZ_CP103550:c5287493-5287110 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT255463 WP_004150355.1 NZ_CP103550:c5287735-5287493 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References