Detailed information of TA system TA025546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2999765..3000479
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: MCAN_RS13890
• Protein ID: WP_003413460.1
• Coordinates: 3000039..3000479 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQJ9
• Locus tag: MCAN_RS13885
• Protein ID: WP_014001348.1
• Coordinates: 2999765..3000052 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS13850	2995187..2995432	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MCAN_RS13855	2995429..2995833	+	405	WP_014001345.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS13860	2996050..2996670	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
MCAN_RS13865	2996681..2997175	+	495	WP_003413444.1	DUF2617 family protein	-
MCAN_RS13870	2997172..2997603	+	432	WP_003413445.1	DUF4247 domain-containing protein	-
MCAN_RS13875	2997628..2998086	+	459	WP_041179710.1	DUF350 domain-containing protein	-
MCAN_RS13880	2998083..2999654	+	1572	WP_014001347.1	polyamine aminopropyltransferase	-
MCAN_RS13885	2999765..3000052	+	288	WP_014001348.1	antitoxin	Antitoxin
MCAN_RS13890	3000039..3000479	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS13895	3000500..3001255	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
MCAN_RS13900	3001388..3001984	-	597	WP_014001349.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
MCAN_RS13905	3001992..3002837	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
MCAN_RS13910	3002866..3003765	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
MCAN_RS13915	3003893..3004567	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T25546 WP_003413460.1 NC_015848:3000039-3000479 [Mycobacterium canettii CIPT 140010059]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T25546 NC_015848:3000039-3000479 [Mycobacterium canettii CIPT 140010059]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTACGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCCGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10514.04 Da
• Isoelectric Point: 7.2797

>AT25546 WP_014001348.1 NC_015848:2999765-3000052 [Mycobacterium canettii CIPT 140010059]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCSGATTREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT25546 NC_015848:2999765-3000052 [Mycobacterium canettii CIPT 140010059]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCAGTGGCGCGACTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: G0TQJ9