Detailed information of TA system TA025545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2939414..2940051
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ29
• Locus tag: MCAN_RS13625
• Protein ID: WP_014001328.1
• Coordinates: 2939414..2939809 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ30
• Locus tag: MCAN_RS13630
• Protein ID: WP_014001329.1
• Coordinates: 2939806..2940051 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS13585	2935499..2936158	+	660	WP_014001324.1	LppA family lipoprotein	-
MCAN_RS13590	2936155..2936814	+	660	WP_014001325.1	hypothetical protein	-
MCAN_RS13595	2936811..2937473	+	663	WP_014001326.1	hypothetical protein	-
MCAN_RS13600	2937470..2937748	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
MCAN_RS13605	2937841..2938254	+	414	WP_003413164.1	PIN domain nuclease	-
MCAN_RS13610	2938293..2938550	+	258	WP_014001327.1	ribbon-helix-helix domain-containing protein	-
MCAN_RS13615	2938547..2938924	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS13620	2938940..2939314	-	375	WP_003413177.1	hypothetical protein	-
MCAN_RS13625	2939414..2939809	-	396	WP_014001328.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
MCAN_RS13630	2939806..2940051	-	246	WP_014001329.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
MCAN_RS13635	2940462..2940881	-	420	WP_014001330.1	A24 family peptidase	-
MCAN_RS13640	2940893..2941702	-	810	WP_014001331.1	shikimate dehydrogenase	-
MCAN_RS13645	2941699..2942952	-	1254	WP_014001332.1	endolytic transglycosylase MltG	-
MCAN_RS13650	2942945..2943457	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14647.54 Da
• Isoelectric Point: 6.6413

>T25545 WP_014001328.1 NC_015848:c2939809-2939414 [Mycobacterium canettii CIPT 140010059]
MIFVDTSFWAALGNAGDARHGTAQRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFVVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T25545 NC_015848:c2939809-2939414 [Mycobacterium canettii CIPT 140010059]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGCAGAGGTTGTG
GGCCAGCAAACCGCCCGTAGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCGTGGGAATGGCTTGTACGTCACGACGAGCGAGAGTACTCGTTCGTCGACGCGACGAGCTTCGTGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9112.36 Da
• Isoelectric Point: 5.6007

>AT25545 WP_014001329.1 NC_015848:c2940051-2939806 [Mycobacterium canettii CIPT 140010059]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASIDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT25545 NC_015848:c2940051-2939806 [Mycobacterium canettii CIPT 140010059]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCATCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• AlphaFold DB: G0TQ29

Antitoxin

• AlphaFold DB: G0TQ30