Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 94065..94590 Replicon plasmid pMB9029_2
Accession NZ_CP103546
Organism Escherichia coli strain 3036

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag M5S54_RS24270 Protein ID WP_001159871.1
Coordinates 94065..94370 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag M5S54_RS24275 Protein ID WP_000813630.1
Coordinates 94372..94590 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S54_RS24245 (90236) 90236..90652 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
M5S54_RS24250 (90645) 90645..91625 - 981 WP_000688514.1 plasmid segregation protein ParM -
M5S54_RS24255 (92039) 92039..92347 - 309 WP_000030199.1 hypothetical protein -
M5S54_RS24260 (92434) 92434..93078 - 645 WP_001144036.1 ParA family protein -
M5S54_RS24265 (93258) 93258..94064 - 807 WP_000016969.1 site-specific integrase -
M5S54_RS24270 (94065) 94065..94370 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
M5S54_RS24275 (94372) 94372..94590 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
M5S54_RS24280 (95148) 95148..96074 + 927 WP_001290416.1 hypothetical protein -
M5S54_RS24285 (96119) 96119..96373 + 255 WP_000678527.1 hypothetical protein -
M5S54_RS24290 (96872) 96872..97216 + 345 WP_000793307.1 hypothetical protein -
M5S54_RS24295 (97392) 97392..98393 - 1002 WP_000774875.1 DUF4238 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 - 1..98998 98998


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T255433 WP_001159871.1 NZ_CP103546:c94370-94065 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT255433 WP_000813630.1 NZ_CP103546:c94590-94372 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References