Detailed information of TA system TA025542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2923359..2923999
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: MCAN_RS13515
• Protein ID: WP_003412970.1
• Coordinates: 2923359..2923778 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: MCAN_RS13520
• Protein ID: WP_003412975.1
• Coordinates: 2923775..2923999 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS13490	2918944..2919666	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
MCAN_RS13495	2920183..2920410	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
MCAN_RS13500	2920407..2920808	+	402	WP_014001314.1	PIN domain-containing protein	-
MCAN_RS13505	2920843..2921763	-	921	WP_014001315.1	restriction endonuclease	-
MCAN_RS22485	2922104..2922349	-	246	WP_145974196.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MCAN_RS13510	2922408..2923358	+	951	WP_015288319.1	Lsr2 family protein	-
MCAN_RS13515	2923359..2923778	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS13520	2923775..2923999	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
MCAN_RS13525	2924030..2926873	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
MCAN_RS13530	2926945..2927346	-	402	WP_003412981.1	hypothetical protein	-
MCAN_RS13535	2927346..2927816	-	471	WP_003412985.1	transcription antitermination factor NusB	-
MCAN_RS13540	2927819..2928382	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T25542 WP_003412970.1 NC_015848:c2923778-2923359 [Mycobacterium canettii CIPT 140010059]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T25542 NC_015848:c2923778-2923359 [Mycobacterium canettii CIPT 140010059]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT25542 WP_003412975.1 NC_015848:c2923999-2923775 [Mycobacterium canettii CIPT 140010059]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT25542 NC_015848:c2923999-2923775 [Mycobacterium canettii CIPT 140010059]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09