Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20592..21235 Replicon plasmid pMB9029_1
Accession NZ_CP103545
Organism Escherichia coli strain 3036

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag M5S54_RS23060 Protein ID WP_001044768.1
Coordinates 20819..21235 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag M5S54_RS23055 Protein ID WP_001261287.1
Coordinates 20592..20822 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S54_RS23035 (15752) 15752..16840 + 1089 WP_000952217.1 transcriptional repressor PifC -
M5S54_RS23040 (16842) 16842..19067 + 2226 WP_000698737.1 P-loop NTPase fold protein -
M5S54_RS23045 (19117) 19117..20016 - 900 WP_000963206.1 nucleotide-binding protein -
M5S54_RS23050 (20006) 20006..20296 - 291 WP_000111771.1 hypothetical protein -
M5S54_RS23055 (20592) 20592..20822 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M5S54_RS23060 (20819) 20819..21235 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
M5S54_RS23065 (21397) 21397..23535 - 2139 WP_000350638.1 AAA family ATPase -
M5S54_RS23070 (23889) 23889..24146 + 258 WP_000343085.1 hypothetical protein -
M5S54_RS23075 (24146) 24146..24736 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / tet(A) / catA2 / sul3 / ant(3'')-Ia / floR / aac(3)-IIa / sitABCD iutA / iucD / iucC / iucB / iucA 1..132824 132824


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T255419 WP_001044768.1 NZ_CP103545:20819-21235 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT255419 WP_001261287.1 NZ_CP103545:20592-20822 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References