255413

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3476629..3477466 Replicon chromosome
Accession NZ_CP103544
Organism Escherichia coli strain 3036

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag M5S54_RS16815 Protein ID WP_000227784.1
Coordinates 3476924..3477466 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag M5S54_RS16810 Protein ID WP_001297137.1
Coordinates 3476629..3476940 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S54_RS16785 (3471649) 3471649..3472596 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
M5S54_RS16790 (3472618) 3472618..3474609 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
M5S54_RS16795 (3474599) 3474599..3475213 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
M5S54_RS16800 (3475213) 3475213..3475542 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
M5S54_RS16805 (3475554) 3475554..3476444 + 891 WP_000971336.1 heme o synthase -
M5S54_RS16810 (3476629) 3476629..3476940 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
M5S54_RS16815 (3476924) 3476924..3477466 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
M5S54_RS16820 (3477522) 3477522..3478457 - 936 WP_001297127.1 tetratricopeptide repeat protein -
M5S54_RS16825 (3478865) 3478865..3480229 + 1365 WP_001000978.1 MFS transporter -
M5S54_RS16830 (3480357) 3480357..3480848 - 492 WP_001138904.1 nucleotide binding protein YajQ -
M5S54_RS16835 (3481016) 3481016..3481927 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T255413 WP_000227784.1 NZ_CP103544:3476924-3477466 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT255413 WP_001297137.1 NZ_CP103544:3476629-3476940 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References