Detailed information of TA system TA025541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2876144..2876796
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPX1
• Locus tag: MCAN_RS13305
• Protein ID: WP_003412752.1
• Coordinates: 2876371..2876796 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPX0
• Locus tag: MCAN_RS13300
• Protein ID: WP_014001300.1
• Coordinates: 2876144..2876365 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS13285	2874384..2874590	-	207	WP_080602906.1	hypothetical protein	-
MCAN_RS13290	2874726..2875349	+	624	WP_014001298.1	TIGR00725 family protein	-
MCAN_RS13295	2875339..2876091	+	753	WP_014001299.1	hypothetical protein	-
MCAN_RS13300	2876144..2876365	+	222	WP_014001300.1	antitoxin	Antitoxin
MCAN_RS13305	2876371..2876796	+	426	WP_003412752.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS13310	2876819..2878000	-	1182	WP_014001301.1	2-oxo acid dehydrogenase subunit E2	-
MCAN_RS13315	2877997..2879043	-	1047	WP_003412757.1	3-methyl-2-oxobutanoate dehydrogenase subunit beta	-
MCAN_RS13320	2879054..2880157	-	1104	WP_003412761.1	pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha	-
MCAN_RS13325	2880416..2881237	-	822	WP_003412766.1	citrate (pro-3S)-lyase subunit beta	-
MCAN_RS13330	2881234..2881791	-	558	WP_014001302.1	MaoC family dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15392.89 Da
• Isoelectric Point: 9.2386

>T25541 WP_003412752.1 NC_015848:2876371-2876796 [Mycobacterium canettii CIPT 140010059]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Nucleotide

• Length: 426 bp

>T25541 NC_015848:2876371-2876796 [Mycobacterium canettii CIPT 140010059]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7878.96 Da
• Isoelectric Point: 4.5258

>AT25541 WP_014001300.1 NC_015848:2876144-2876365 [Mycobacterium canettii CIPT 140010059]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRIEADDGLPVIRVPAGTPPITPEMVRRALDED

Nucleotide

• Length: 222 bp

>AT25541 NC_015848:2876144-2876365 [Mycobacterium canettii CIPT 140010059]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCATCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Toxin

• AlphaFold DB: G0TPX1

Antitoxin

• AlphaFold DB: G0TPX0