Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4043541..4044117 Replicon chromosome
Accession NZ_CP103540
Organism Escherichia coli strain 5264

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5S66_RS19555 Protein ID WP_001297643.1
Coordinates 4043830..4044117 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag M5S66_RS19550 Protein ID WP_000063160.1
Coordinates 4043541..4043843 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S66_RS19535 (4040137) 4040137..4042287 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5S66_RS19540 (4042337) 4042337..4042540 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5S66_RS19545 (4042551) 4042551..4043507 + 957 WP_001297640.1 GTPase -
M5S66_RS19550 (4043541) 4043541..4043843 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
M5S66_RS19555 (4043830) 4043830..4044117 - 288 WP_001297643.1 BrnT family toxin Toxin
M5S66_RS19560 (4044316) 4044316..4045012 - 697 Protein_3820 IS1 family transposase -
M5S66_RS19565 (4045327) 4045327..4046559 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
M5S66_RS19570 (4046600) 4046600..4047880 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4044316..4044525 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T255393 WP_001297643.1 NZ_CP103540:c4044117-4043830 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT255393 WP_000063160.1 NZ_CP103540:c4043843-4043541 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References