Detailed information of TA system TA025539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2419365..2420060
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TMM9
• Locus tag: MCAN_RS11135
• Protein ID: WP_014001135.1
• Coordinates: 2419365..2419799 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TMN0
• Locus tag: MCAN_RS11140
• Protein ID: WP_003410814.1
• Coordinates: 2419806..2420060 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS11125	2415522..2418563	+	3042	WP_014001133.1	DEAD/DEAH box helicase	-
MCAN_RS11130	2418556..2419386	+	831	WP_041180101.1	SWIM zinc finger family protein	-
MCAN_RS11135	2419365..2419799	-	435	WP_014001135.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS11140	2419806..2420060	-	255	WP_003410814.1	antitoxin	Antitoxin
MCAN_RS11145	2420076..2420333	-	258	WP_041180102.1	hypothetical protein	-
MCAN_RS11150	2421277..2421573	+	297	WP_014001136.1	PE family protein	-
MCAN_RS11155	2421629..2422360	+	732	WP_014001137.1	PPE family protein	-
MCAN_RS11165	2422902..2423648	-	747	WP_003901330.1	proteasome subunit alpha	-
MCAN_RS11170	2423645..2424520	-	876	WP_014001138.1	proteasome subunit beta	-
MCAN_RS11175	2424517..2424711	-	195	WP_003411026.1	ubiquitin-like protein Pup	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15620.04 Da
• Isoelectric Point: 8.6024

>T25539 WP_014001135.1 NC_015848:c2419799-2419365 [Mycobacterium canettii CIPT 140010059]
MKIVDANVLLYAVNTTSEHHKPSLRWLDGALSGADRVGFAWVPLLSFVRLATKVGLFPRPLPREAAITQVADWLAAPSAV
LVNPTVRHADILARMLTYVGTGANLVNDAHLAALAVEHRASIVSYDSDFGRFAGVRWDQPPALL

Nucleotide

• Length: 435 bp

>T25539 NC_015848:c2419799-2419365 [Mycobacterium canettii CIPT 140010059]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGTCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTCGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGCAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9417.57 Da
• Isoelectric Point: 8.9912

>AT25539 WP_003410814.1 NC_015848:c2420060-2419806 [Mycobacterium canettii CIPT 140010059]
MRTTVTLDDDVEQLVRRRMAERQVSFKKALNDAIRDGASGRPAPSHFSTRTADLGVPAVNLDRALQLAADLEDEELVRRQ
RRGS

Nucleotide

• Length: 255 bp

>AT25539 NC_015848:c2420060-2419806 [Mycobacterium canettii CIPT 140010059]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Toxin

• AlphaFold DB: G0TMM9

Antitoxin

• AlphaFold DB: G0TMN0