Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 860323..860977 Replicon chromosome
Accession NZ_CP103540
Organism Escherichia coli strain 5264

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag M5S66_RS04215 Protein ID WP_000244781.1
Coordinates 860570..860977 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag M5S66_RS04210 Protein ID WP_000354046.1
Coordinates 860323..860589 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S66_RS04190 (856411) 856411..857844 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
M5S66_RS04195 (857889) 857889..858200 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
M5S66_RS04200 (858364) 858364..859023 + 660 WP_000250269.1 hemolysin III family protein -
M5S66_RS04205 (859100) 859100..860080 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
M5S66_RS04210 (860323) 860323..860589 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
M5S66_RS04215 (860570) 860570..860977 + 408 WP_000244781.1 protein YgfX Toxin
M5S66_RS04220 (861017) 861017..861538 - 522 WP_001521233.1 flavodoxin FldB -
M5S66_RS04225 (861650) 861650..862546 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
M5S66_RS04230 (862571) 862571..863281 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
M5S66_RS04235 (863287) 863287..865020 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T255381 WP_000244781.1 NZ_CP103540:860570-860977 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT255381 WP_000354046.1 NZ_CP103540:860323-860589 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References