Detailed information of TA system TA025538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2370563..2371199
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MCAN_RS10900
• Protein ID: WP_015288230.1
• Coordinates: 2370789..2371199 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TM57
• Locus tag: MCAN_RS10895
• Protein ID: WP_014001104.1
• Coordinates: 2370563..2370796 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS10885	2366413..2366817	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
MCAN_RS10890	2366901..2370485	-	3585	WP_014001103.1	cobaltochelatase subunit CobN	-
MCAN_RS10895	2370563..2370796	+	234	WP_014001104.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
MCAN_RS10900	2370789..2371199	+	411	WP_015288230.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
MCAN_RS10905	2371183..2372274	+	1092	WP_014001105.1	precorrin-3B synthase	-
MCAN_RS10910	2372284..2372910	+	627	WP_014001106.1	precorrin-8X methylmutase	-
MCAN_RS10915	2372907..2374433	+	1527	WP_014001107.1	precorrin-2 C(20)-methyltransferase	-
MCAN_RS10920	2374379..2375602	-	1224	WP_014001108.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14221.39 Da
• Isoelectric Point: 10.6228

>T25538 WP_015288230.1 NC_015848:2370789-2371199 [Mycobacterium canettii CIPT 140010059]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLIVGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T25538 NC_015848:2370789-2371199 [Mycobacterium canettii CIPT 140010059]
TTGGCTGAGCCACGGCGGGGAGACCTTTGGCTAGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCGTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCAACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8430.25 Da
• Isoelectric Point: 4.7954

>AT25538 WP_014001104.1 NC_015848:2370563-2370796 [Mycobacterium canettii CIPT 140010059]
VSTSTTIRVSIQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT25538 NC_015848:2370563-2370796 [Mycobacterium canettii CIPT 140010059]
GTGTCTACATCCACGACGATTAGGGTGTCAATCCAGACTCGGGACCGTCTGGCCGCCCAAGCCCGCGAACGAGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCACACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: G0TM57