Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 68869..69428 Replicon plasmid pMB9108_2
Accession NZ_CP103535
Organism Escherichia coli strain 4238

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag M5T38_RS24295 Protein ID WP_000421257.1
Coordinates 69153..69428 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag M5T38_RS24290 Protein ID WP_001178089.1
Coordinates 68869..69153 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T38_RS24270 (M5T38_24270) 63898..64518 - 621 WP_000176497.1 DUF1449 family protein -
M5T38_RS24275 (M5T38_24275) 64785..65447 - 663 WP_000154142.1 hypothetical protein -
M5T38_RS24280 (M5T38_24280) 65588..66229 - 642 WP_000335849.1 transcription termination/antitermination NusG family protein -
M5T38_RS24285 (M5T38_24285) 66930..67961 + 1032 WP_000907880.1 plasmid replication initiator RepA -
M5T38_RS24290 (M5T38_24290) 68869..69153 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
M5T38_RS24295 (M5T38_24295) 69153..69428 + 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T38_RS24300 (M5T38_24300) 69534..69737 + 204 Protein_73 hypothetical protein -
M5T38_RS24305 (M5T38_24305) 70006..70272 - 267 WP_000627831.1 hypothetical protein -
M5T38_RS24310 (M5T38_24310) 70510..71112 - 603 WP_000517694.1 ProQ/FINO family protein -
M5T38_RS24315 (M5T38_24315) 71466..72077 + 612 Protein_76 hypothetical protein -
M5T38_RS24320 (M5T38_24320) 72225..73085 - 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
M5T38_RS24325 (M5T38_24325) 73268..73825 - 558 WP_001235713.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..94558 94558


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T255376 WP_000421257.1 NZ_CP103535:69153-69428 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT255376 WP_001178089.1 NZ_CP103535:68869-69153 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References