25536

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2305204..2305830 Replicon chromosome
Accession NC_015848
Organism Mycobacterium canettii CIPT 140010059

Toxin (Protein)


Gene name vapC Uniprot ID G0TM01
Locus tag MCAN_RS10605 Protein ID WP_014001061.1
Coordinates 2305432..2305830 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag MCAN_RS10600 Protein ID WP_003911750.1
Coordinates 2305204..2305431 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCAN_RS10590 2303242..2303586 - 345 WP_003410065.1 ferredoxin family protein -
MCAN_RS10595 2303775..2304944 - 1170 WP_003899126.1 ATP-binding protein -
MCAN_RS10600 2305204..2305431 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MCAN_RS10605 2305432..2305830 + 399 WP_014001061.1 PIN domain nuclease Toxin
MCAN_RS10610 2306032..2306463 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
MCAN_RS10615 2306564..2306983 + 420 WP_178125312.1 DUF1398 domain-containing protein -
MCAN_RS21460 2306980..2307229 + 250 Protein_2087 hypothetical protein -
MCAN_RS22700 2307257..2307409 + 153 WP_157186096.1 hypothetical protein -
MCAN_RS10620 2307486..2308397 + 912 Protein_2089 IS21 family transposase -
MCAN_RS10625 2308399..2309151 + 753 WP_041180092.1 IS21-like element helper ATPase IstB -
MCAN_RS22155 2309245..2309424 - 180 Protein_2091 hypothetical protein -
MCAN_RS23065 2309735..2310198 + 464 Protein_2092 transposase -
MCAN_RS23070 2310254..2310763 + 510 WP_145974195.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14777.98 Da        Isoelectric Point: 5.6825

>T25536 WP_014001061.1 NC_015848:2305432-2305830 [Mycobacterium canettii CIPT 140010059]
MIVDTSVWIEYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGTTDEDSAALRRRLLQRFTIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T25536 NC_015848:2305432-2305830 [Mycobacterium canettii CIPT 140010059]
ATGATCGTCGACACCTCGGTCTGGATCGAATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTACGACCGATGAGGACAGCGCCG
CACTGCGCCGACGGCTCCTGCAACGATTCACTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT25536 WP_003911750.1 NC_015848:2305204-2305431 [Mycobacterium canettii CIPT 140010059]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT25536 NC_015848:2305204-2305431 [Mycobacterium canettii CIPT 140010059]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TM01

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References