Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 350724..351524 Replicon chromosome
Accession NZ_CP103533
Organism Escherichia coli strain 4238

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag M5T38_RS01630 Protein ID WP_000342449.1
Coordinates 350997..351524 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag M5T38_RS01625 Protein ID WP_001277108.1
Coordinates 350724..350990 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T38_RS01605 (346381) 346381..347049 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
M5T38_RS01610 (347042) 347042..348100 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
M5T38_RS01615 (348345) 348345..349199 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
M5T38_RS01620 (349471) 349471..350574 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
M5T38_RS01625 (350724) 350724..350990 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
M5T38_RS01630 (350997) 350997..351524 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
M5T38_RS01635 (351521) 351521..351904 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
M5T38_RS01640 (352328) 352328..353437 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
M5T38_RS01645 (353485) 353485..354411 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
M5T38_RS01650 (354408) 354408..355685 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
M5T38_RS01655 (355682) 355682..356449 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T255352 WP_000342449.1 NZ_CP103533:350997-351524 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT255352 WP_001277108.1 NZ_CP103533:350724-350990 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References