Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2281465..2282051 Replicon chromosome
Accession NC_015848
Organism Mycobacterium canettii CIPT 140010059

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag MCAN_RS10500 Protein ID WP_003410010.1
Coordinates 2281465..2281809 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag MCAN_RS10505 Protein ID WP_003410014.1
Coordinates 2281803..2282051 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCAN_RS10470 2277179..2277409 + 231 WP_003409980.1 DUF167 domain-containing protein -
MCAN_RS10475 2277494..2278405 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
MCAN_RS10480 2278514..2279113 + 600 WP_003409989.1 L-lysine exporter -
MCAN_RS10485 2279529..2279957 + 429 WP_014001048.1 cellulose-binding protein -
MCAN_RS10490 2280183..2280722 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
MCAN_RS10495 2281253..2281396 - 144 WP_015288198.1 hypothetical protein -
MCAN_RS10500 2281465..2281809 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
MCAN_RS10505 2281803..2282051 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
MCAN_RS10510 2282151..2284466 - 2316 WP_014001049.1 cation transporter ATPase CptG -
MCAN_RS10515 2284463..2284735 - 273 WP_014001050.1 DUF1490 family protein -
MCAN_RS10520 2284788..2285144 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
MCAN_RS10525 2285301..2286068 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T25535 WP_003410010.1 NC_015848:c2281809-2281465 [Mycobacterium canettii CIPT 140010059]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T25535 NC_015848:c2281809-2281465 [Mycobacterium canettii CIPT 140010059]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT25535 WP_003410014.1 NC_015848:c2282051-2281803 [Mycobacterium canettii CIPT 140010059]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT25535 NC_015848:c2282051-2281803 [Mycobacterium canettii CIPT 140010059]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References