Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4279728..4280304 Replicon chromosome
Accession NZ_CP103529
Organism Escherichia coli strain 5270

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5T24_RS20655 Protein ID WP_001297643.1
Coordinates 4280017..4280304 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag M5T24_RS20650 Protein ID WP_000063160.1
Coordinates 4279728..4280030 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T24_RS20635 (4276324) 4276324..4278474 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5T24_RS20640 (4278524) 4278524..4278727 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5T24_RS20645 (4278738) 4278738..4279694 + 957 WP_001297640.1 GTPase -
M5T24_RS20650 (4279728) 4279728..4280030 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
M5T24_RS20655 (4280017) 4280017..4280304 - 288 WP_001297643.1 BrnT family toxin Toxin
M5T24_RS20660 (4280503) 4280503..4281199 - 697 Protein_4043 IS1 family transposase -
M5T24_RS20665 (4281514) 4281514..4282746 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
M5T24_RS20670 (4282787) 4282787..4284067 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4280503..4280712 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T255343 WP_001297643.1 NZ_CP103529:c4280304-4280017 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT255343 WP_000063160.1 NZ_CP103529:c4280030-4279728 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References