Detailed information of TA system TA025533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2273916..2274604
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TLX6
• Locus tag: MCAN_RS10450
• Protein ID: WP_014001046.1
• Coordinates: 2273916..2274335 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MCAN_RS10455
• Protein ID: WP_015288196.1
• Coordinates: 2274344..2274604 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS10430	2269411..2270259	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
MCAN_RS10435	2270222..2271667	-	1446	WP_014001043.1	APC family permease	-
MCAN_RS10440	2271846..2272532	-	687	WP_014001044.1	immunoprotective protein Mpt64	-
MCAN_RS10445	2272723..2273691	-	969	WP_014001045.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
MCAN_RS10450	2273916..2274335	-	420	WP_014001046.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS10455	2274344..2274604	-	261	WP_015288196.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MCAN_RS10460	2274747..2276423	+	1677	WP_014001047.1	PecA family PE domain-processing aspartic protease	-
MCAN_RS10465	2276411..2277064	-	654	WP_003409976.1	cutinase Cfp21	-
MCAN_RS10470	2277179..2277409	+	231	WP_003409980.1	DUF167 domain-containing protein	-
MCAN_RS10475	2277494..2278405	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
MCAN_RS10480	2278514..2279113	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14702.84 Da
• Isoelectric Point: 7.3842

>T25533 WP_014001046.1 NC_015848:c2274335-2273916 [Mycobacterium canettii CIPT 140010059]
MIVDTSAVVALVQGERPHATLVAAALAGADSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T25533 NC_015848:c2274335-2273916 [Mycobacterium canettii CIPT 140010059]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCGATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGAGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9558.91 Da
• Isoelectric Point: 5.1007

>AT25533 WP_015288196.1 NC_015848:c2274604-2274344 [Mycobacterium canettii CIPT 140010059]
VALNIKDPEVDRLATELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT25533 NC_015848:c2274604-2274344 [Mycobacterium canettii CIPT 140010059]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCACCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: G0TLX6