Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 74165..74808 Replicon plasmid pMB9272_2
Accession NZ_CP103523
Organism Escherichia coli strain 2779 - blood draw same patient as MB9267

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag M5S63_RS25690 Protein ID WP_001044768.1
Coordinates 74165..74581 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag M5S63_RS25695 Protein ID WP_001261287.1
Coordinates 74578..74808 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S63_RS25675 (69469) 69469..70401 - 933 WP_000991832.1 S-4TM family putative pore-forming effector -
M5S63_RS25680 (70405) 70405..71400 - 996 WP_000246636.1 hypothetical protein -
M5S63_RS25685 (71865) 71865..74003 + 2139 WP_000350635.1 AAA family ATPase -
M5S63_RS25690 (74165) 74165..74581 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
M5S63_RS25695 (74578) 74578..74808 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M5S63_RS25700 (75115) 75115..78234 + 3120 WP_023909028.1 hypothetical protein -
M5S63_RS25705 (78497) 78497..79630 + 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / sul1 / qacE / aadA5 / tet(B) - 1..82867 82867
- flank IS/Tn - - 68829..69332 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T255323 WP_001044768.1 NZ_CP103523:c74581-74165 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT255323 WP_001261287.1 NZ_CP103523:c74808-74578 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References