Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 61575..62176 Replicon plasmid pMB9272_2
Accession NZ_CP103523
Organism Escherichia coli strain 2779 - blood draw same patient as MB9267

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag M5S63_RS25645 Protein ID WP_001216034.1
Coordinates 61575..61955 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag M5S63_RS25650 Protein ID WP_001190712.1
Coordinates 61955..62176 (-) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S63_RS25605 (56955) 56955..57223 + 269 Protein_73 type II toxin-antitoxin system ParD family antitoxin -
M5S63_RS25610 (57210) 57210..57499 + 290 Protein_74 type II toxin-antitoxin system RelE/ParE family toxin -
M5S63_RS25615 (57566) 57566..57748 + 183 WP_042065278.1 hypothetical protein -
M5S63_RS25620 (57869) 57869..58609 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -
M5S63_RS25625 (58894) 58894..59871 - 978 WP_000361610.1 RepB family plasmid replication initiator protein -
M5S63_RS25630 (60667) 60667..61080 - 414 Protein_78 integrase core domain-containing protein -
M5S63_RS25635 (61085) 61085..61363 - 279 Protein_79 pdcB -
M5S63_RS25640 (61391) 61391..61570 - 180 WP_001513661.1 hypothetical protein -
M5S63_RS25645 (61575) 61575..61955 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
M5S63_RS25650 (61955) 61955..62176 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M5S63_RS25655 (62359) 62359..63915 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
M5S63_RS25660 (63912) 63912..65183 + 1272 WP_023142242.1 restriction endonuclease subunit S -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / sul1 / qacE / aadA5 / tet(B) - 1..82867 82867


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T255322 WP_001216034.1 NZ_CP103523:c61955-61575 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp


Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT255322 WP_001190712.1 NZ_CP103523:c62176-61955 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0AJ64


Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References