Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4629572..4630069 Replicon chromosome
Accession NZ_CP103521
Organism Escherichia coli strain 2779 - blood draw same patient as MB9267

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5S63_RS22520 Protein ID WP_001173343.1
Coordinates 4629572..4629745 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M5S63_RS22525 Protein ID WP_000398619.1
Coordinates 4629773..4630069 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S63_RS22510 (4626242) 4626242..4627699 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
M5S63_RS22515 (4627936) 4627936..4629453 + 1518 WP_000003806.1 lysine--tRNA ligase -
M5S63_RS22520 (4629572) 4629572..4629745 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5S63_RS22525 (4629773) 4629773..4630069 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5S63_RS22530 (4630298) 4630298..4630570 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5S63_RS22535 (4630582) 4630582..4630812 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
M5S63_RS22540 (4630993) 4630993..4632624 + 1632 WP_001216477.1 sensor histidine kinase -
M5S63_RS22545 (4632621) 4632621..4633340 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4584269..4630069 45800


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T255312 WP_001173343.1 NZ_CP103521:c4629745-4629572 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT255312 WP_000398619.1 NZ_CP103521:c4630069-4629773 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References