Detailed information of TA system TA255306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3938182..3939871
• Replicon: chromosome
• Accession: NZ_CP103521
• Organism: Escherichia coli strain 2779 - blood draw same patient as MB9267

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: M5S63_RS19125
• Protein ID: WP_001521912.1
• Coordinates: 3938182..3939102 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5S63_RS19130
• Protein ID: WP_001521911.1
• Coordinates: 3939089..3939871 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5S63_RS19110 (3934272)	3934272..3935399	-	1128	WP_001521913.1	MFS transporter	-
M5S63_RS19115 (3935541)	3935541..3936599	-	1059	WP_122452191.1	alpha/beta hydrolase	-
M5S63_RS19120 (3936864)	3936864..3937748	+	885	WP_001119037.1	LysR family transcriptional regulator	-
M5S63_RS19125 (3938182)	3938182..3939102	-	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5S63_RS19130 (3939089)	3939089..3939871	-	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5S63_RS19135 (3940187)	3940187..3943396	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
M5S63_RS19140 (3943743)	3943743..3944213	+	471	WP_000201185.1	hypothetical protein	-
M5S63_RS19145 (3944210)	3944210..3944312	+	103	Protein_3749	helix-turn-helix domain-containing protein	-
M5S63_RS19150 (3944404)	3944404..3944562	+	159	Protein_3750	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T255306 WP_001521912.1 NZ_CP103521:c3939102-3938182 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT255306 WP_001521911.1 NZ_CP103521:c3939871-3939089 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A829L165