Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3028989..3029773 Replicon chromosome
Accession NZ_CP103521
Organism Escherichia coli strain 2779 - blood draw same patient as MB9267

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M5S63_RS14905 Protein ID WP_000613626.1
Coordinates 3029279..3029773 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag M5S63_RS14900 Protein ID WP_001110447.1
Coordinates 3028989..3029282 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S63_RS14890 (3024139) 3024139..3025098 - 960 WP_000846343.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
M5S63_RS14895 (3025671) 3025671..3028856 + 3186 WP_001522867.1 ribonuclease E -
M5S63_RS14900 (3028989) 3028989..3029282 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
M5S63_RS14905 (3029279) 3029279..3029773 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M5S63_RS14910 (3029868) 3029868..3030821 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
M5S63_RS14915 (3030833) 3030833..3032476 - 1644 WP_001522865.1 flagellar hook-associated protein FlgK -
M5S63_RS14920 (3032542) 3032542..3033483 - 942 WP_001522863.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M5S63_RS14925 (3033483) 3033483..3034580 - 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T255303 WP_000613626.1 NZ_CP103521:3029279-3029773 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT255303 WP_001110447.1 NZ_CP103521:3028989-3029282 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References