Detailed information of TA system TA025530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2238651..2239354
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: MCAN_RS10210
• Protein ID: WP_003409778.1
• Coordinates: 2238651..2238980 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: MCAN_RS21430
• Protein ID: WP_003409780.1
• Coordinates: 2238977..2239354 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS10190	2235034..2236104	+	1071	WP_014001022.1	epoxide hydrolase EphB	-
MCAN_RS10195	2236101..2236616	+	516	WP_003409718.1	flavin reductase family protein	-
MCAN_RS10200	2236613..2237674	+	1062	WP_014001023.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MCAN_RS10205	2237671..2238441	+	771	WP_003409775.1	SDR family oxidoreductase	-
MCAN_RS10210	2238651..2238980	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MCAN_RS21430	2238977..2239354	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
MCAN_RS10220	2239351..2239941	-	591	WP_014001024.1	SEC-C domain-containing protein	-
MCAN_RS10225	2239996..2241360	+	1365	WP_009939438.1	HNH endonuclease	-
MCAN_RS10230	2241515..2241967	-	453	WP_003899095.1	lipoprotein	-
MCAN_RS10235	2242031..2242432	+	402	WP_003409869.1	hypothetical protein	-
MCAN_RS10240	2242425..2242607	-	183	WP_003409870.1	hypothetical protein	-
MCAN_RS10245	2242721..2243071	-	351	WP_003409871.1	hypothetical protein	-
MCAN_RS10250	2243082..2243984	-	903	WP_080603716.1	hypothetical protein	-
MCAN_RS21435	2244005..2244197	-	193	Protein_2013	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T25530 WP_003409778.1 NC_015848:c2238980-2238651 [Mycobacterium canettii CIPT 140010059]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T25530 NC_015848:c2238980-2238651 [Mycobacterium canettii CIPT 140010059]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT25530 WP_003409780.1 NC_015848:c2239354-2238977 [Mycobacterium canettii CIPT 140010059]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT25530 NC_015848:c2239354-2238977 [Mycobacterium canettii CIPT 140010059]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8