Detailed information of TA system TA025529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2001963..2002423
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TKL9
• Locus tag: MCAN_RS09185
• Protein ID: WP_003898996.1
• Coordinates: 2002175..2002423 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TKL8
• Locus tag: MCAN_RS09180
• Protein ID: WP_014000915.1
• Coordinates: 2001963..2002175 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS09165	1998441..1999628	-	1188	WP_014000913.1	nitrate transporter NarK	-
MCAN_RS09170	1999915..2000199	+	285	WP_003408522.1	DUF1876 domain-containing protein	-
MCAN_RS09175	2000213..2001895	-	1683	WP_014000914.1	SulP family inorganic anion transporter	-
MCAN_RS09180	2001963..2002175	+	213	WP_014000915.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MCAN_RS09185	2002175..2002423	+	249	WP_003898996.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS09190	2002431..2003168	+	738	WP_014000916.1	hypothetical protein	-
MCAN_RS09195	2003262..2004962	+	1701	WP_014000917.1	serine/threonine protein kinase PknE	-
MCAN_RS09200	2005247..2005648	-	402	WP_014000918.1	hypothetical protein	-
MCAN_RS09205	2005638..2006249	-	612	WP_003898999.1	isopentenyl-diphosphate Delta-isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin VapB-like (2-69)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8884.14 Da
• Isoelectric Point: 4.1074

>T25529 WP_003898996.1 NC_015848:2002175-2002423 [Mycobacterium canettii CIPT 140010059]
MVIDTSALVAMLNDEPEAQRFEIAVAADHVWLMSTASYPEMATVIETRFGEPGGREPKVSGQPLLYKGDDFACIDIRAVL
AG

Nucleotide

• Length: 249 bp

>T25529 NC_015848:2002175-2002423 [Mycobacterium canettii CIPT 140010059]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7604.78 Da
• Isoelectric Point: 10.3801

>AT25529 WP_014000915.1 NC_015848:2001963-2002175 [Mycobacterium canettii CIPT 140010059]
MELAARTGETLTQAVVVAVREQLARRTGRTRSISLREELAAIGRRCAALPVLDTRAADKILGYDERGLPA

Nucleotide

• Length: 213 bp

>AT25529 NC_015848:2001963-2002175 [Mycobacterium canettii CIPT 140010059]
ATGGAATTGGCGGCTCGAACGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATCGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACAAGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: G0TKL9

Antitoxin

• AlphaFold DB: G0TKL8