Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3997350..3997926 Replicon chromosome
Accession NZ_CP103515
Organism Escherichia coli strain 5444

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5S96_RS19535 Protein ID WP_001297643.1
Coordinates 3997639..3997926 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5S96_RS19530 Protein ID WP_000063156.1
Coordinates 3997350..3997652 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S96_RS19515 (3993946) 3993946..3996096 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5S96_RS19520 (3996146) 3996146..3996349 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5S96_RS19525 (3996360) 3996360..3997316 + 957 WP_001297640.1 GTPase -
M5S96_RS19530 (3997350) 3997350..3997652 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5S96_RS19535 (3997639) 3997639..3997926 - 288 WP_001297643.1 BrnT family toxin Toxin
M5S96_RS19540 (3998125) 3998125..3998822 - 698 Protein_3819 IS1 family transposase -
M5S96_RS19545 (3998879) 3998879..3998974 + 96 Protein_3820 dienelactone hydrolase family protein -
M5S96_RS19550 (3999120) 3999120..4000352 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
M5S96_RS19555 (4000393) 4000393..4001673 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T255277 WP_001297643.1 NZ_CP103515:c3997926-3997639 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT255277 WP_000063156.1 NZ_CP103515:c3997652-3997350 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References