Detailed information of TA system TA025526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1597363..1598018
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIK1
• Locus tag: MCAN_RS07440
• Protein ID: WP_014000749.1
• Coordinates: 1597363..1597764 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TIK2
• Locus tag: MCAN_RS07445
• Protein ID: WP_003407272.1
• Coordinates: 1597761..1598018 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS07425	1592925..1594310	-	1386	WP_014000746.1	cytochrome P450	-
MCAN_RS07430	1594388..1595422	+	1035	WP_014000747.1	AraC family transcriptional regulator	-
MCAN_RS07435	1595468..1597108	-	1641	WP_014000748.1	PE family protein	-
MCAN_RS07440	1597363..1597764	-	402	WP_014000749.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS07445	1597761..1598018	-	258	WP_003407272.1	ribbon-helix-helix domain-containing protein	Antitoxin
MCAN_RS07450	1598101..1599060	-	960	WP_003407276.1	alpha/beta hydrolase	-
MCAN_RS07455	1599085..1600047	-	963	WP_014000750.1	alpha/beta hydrolase	-
MCAN_RS07460	1600046..1600783	+	738	WP_041180243.1	lysoplasmalogenase	-
MCAN_RS07465	1600864..1602831	+	1968	WP_003407285.1	primosomal protein N'	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14864.37 Da
• Isoelectric Point: 11.8356

>T25526 WP_014000749.1 NC_015848:c1597764-1597363 [Mycobacterium canettii CIPT 140010059]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVRHFPMFEQLKPPFAVPGHRPRA

Nucleotide

• Length: 402 bp

>T25526 NC_015848:c1597764-1597363 [Mycobacterium canettii CIPT 140010059]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGCGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9443.58 Da
• Isoelectric Point: 6.4631

>AT25526 WP_003407272.1 NC_015848:c1598018-1597761 [Mycobacterium canettii CIPT 140010059]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Nucleotide

• Length: 258 bp

>AT25526 NC_015848:c1598018-1597761 [Mycobacterium canettii CIPT 140010059]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Toxin

• AlphaFold DB: G0TIK1

Antitoxin

• AlphaFold DB: G0TIK2