Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4746279..4747089 Replicon chromosome
Accession NZ_CP103512
Organism Klebsiella pneumoniae strain 5531

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag M5S57_RS23020 Protein ID WP_014908042.1
Coordinates 4746279..4746812 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M5S57_RS23025 Protein ID WP_002887278.1
Coordinates 4746823..4747089 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S57_RS23015 (4745110) 4745110..4746231 + 1122 WP_012737335.1 cupin domain-containing protein -
M5S57_RS23020 (4746279) 4746279..4746812 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
M5S57_RS23025 (4746823) 4746823..4747089 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M5S57_RS23030 (4747192) 4747192..4748625 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
M5S57_RS23035 (4748615) 4748615..4749298 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M5S57_RS23040 (4749470) 4749470..4750855 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
M5S57_RS23045 (4750873) 4750873..4751217 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T255254 WP_014908042.1 NZ_CP103512:c4746812-4746279 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT255254 WP_002887278.1 NZ_CP103512:c4747089-4746823 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References