Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 105525..106050 Replicon plasmid pMB9366_1
Accession NZ_CP103509
Organism Escherichia coli O25b:H4-ST131 strain 5506

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag M5T03_RS25145 Protein ID WP_001159868.1
Coordinates 105525..105830 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag M5T03_RS25150 Protein ID WP_000813634.1
Coordinates 105832..106050 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T03_RS25130 (101435) 101435..102601 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
M5T03_RS25135 (103189) 103189..103944 - 756 WP_000852146.1 replication initiation protein RepE -
M5T03_RS25140 (104718) 104718..105524 - 807 WP_000016982.1 site-specific integrase -
M5T03_RS25145 (105525) 105525..105830 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
M5T03_RS25150 (105832) 105832..106050 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
M5T03_RS25155 (106758) 106758..107753 + 996 WP_000246636.1 hypothetical protein -
M5T03_RS25160 (107796) 107796..108410 + 615 WP_259425960.1 S-4TM family putative pore-forming effector -
M5T03_RS25170 (109249) 109249..109407 + 159 WP_228955855.1 abortive infection family protein -
M5T03_RS25175 (109566) 109566..110537 - 972 Protein_133 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-52B senB 1..111292 111292
- flank IS/Tn - - 108404..108907 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T255243 WP_001159868.1 NZ_CP103509:c105830-105525 [Escherichia coli O25b:H4-ST131]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT255243 WP_000813634.1 NZ_CP103509:c106050-105832 [Escherichia coli O25b:H4-ST131]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References