Detailed information of TA system TA025524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1408772..1409331
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: MCAN_RS06625
• Protein ID: WP_003898789.1
• Coordinates: 1408772..1409065 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: MCAN_RS06630
• Protein ID: WP_003406322.1
• Coordinates: 1409062..1409331 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS06600	1404370..1404630	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
MCAN_RS06605	1404627..1405058	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS06610	1405081..1406769	-	1689	WP_014000676.1	PE family protein	-
MCAN_RS06615	1406944..1407804	+	861	WP_014000677.1	hypothetical protein	-
MCAN_RS06620	1407885..1408715	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
MCAN_RS06625	1408772..1409065	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
MCAN_RS06630	1409062..1409331	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MCAN_RS06635	1409500..1413195	-	3696	WP_003406323.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
MCAN_RS06640	1413337..1414125	-	789	WP_014000678.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T25524 WP_003898789.1 NC_015848:c1409065-1408772 [Mycobacterium canettii CIPT 140010059]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T25524 NC_015848:c1409065-1408772 [Mycobacterium canettii CIPT 140010059]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT25524 WP_003406322.1 NC_015848:c1409331-1409062 [Mycobacterium canettii CIPT 140010059]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT25524 NC_015848:c1409331-1409062 [Mycobacterium canettii CIPT 140010059]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTCGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2