Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4160104..4160680 Replicon chromosome
Accession NZ_CP103508
Organism Escherichia coli O25b:H4-ST131 strain 5506

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag M5T03_RS20480 Protein ID WP_001295743.1
Coordinates 4160393..4160680 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag M5T03_RS20475 Protein ID WP_000063148.1
Coordinates 4160104..4160406 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T03_RS20460 (4156700) 4156700..4158850 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5T03_RS20465 (4158900) 4158900..4159103 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5T03_RS20470 (4159114) 4159114..4160070 + 957 WP_001295745.1 GTPase -
M5T03_RS20475 (4160104) 4160104..4160406 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
M5T03_RS20480 (4160393) 4160393..4160680 - 288 WP_001295743.1 BrnT family toxin Toxin
M5T03_RS20485 (4160877) 4160877..4162043 + 1167 WP_000800831.1 restriction endonuclease -
M5T03_RS20490 (4162107) 4162107..4163726 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
M5T03_RS20495 (4163716) 4163716..4165020 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T255231 WP_001295743.1 NZ_CP103508:c4160680-4160393 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT255231 WP_000063148.1 NZ_CP103508:c4160406-4160104 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References