Detailed information of TA system TA255229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3781164..3782853
• Replicon: chromosome
• Accession: NZ_CP103508
• Organism: Escherichia coli O25b:H4-ST131 strain 5506

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: M5T03_RS18655
• Protein ID: WP_000365807.1
• Coordinates: 3781164..3782084 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5T03_RS18660
• Protein ID: WP_001295795.1
• Coordinates: 3782071..3782853 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5T03_RS18615 (3776637)	3776637..3777347	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
M5T03_RS18620 (3777654)	3777654..3777983	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
M5T03_RS18625 (3777995)	3777995..3778161	-	167	Protein_3654	tail fiber assembly protein	-
M5T03_RS18630 (3778232)	3778232..3778846	-	615	WP_001019923.1	YagU family protein	-
M5T03_RS18635 (3779061)	3779061..3779246	-	186	WP_000146240.1	hypothetical protein	-
M5T03_RS18640 (3779295)	3779295..3779453	+	159	Protein_3657	integrase	-
M5T03_RS18645 (3779680)	3779680..3780015	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
M5T03_RS18650 (3780223)	3780223..3780999	+	777	WP_000171155.1	site-specific integrase	-
M5T03_RS18655 (3781164)	3781164..3782084	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5T03_RS18660 (3782071)	3782071..3782853	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5T03_RS18665 (3783137)	3783137..3786235	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3740947..3789916	48969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T255229 WP_000365807.1 NZ_CP103508:c3782084-3781164 [Escherichia coli O25b:H4-ST131]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT255229 WP_001295795.1 NZ_CP103508:c3782853-3782071 [Escherichia coli O25b:H4-ST131]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0