Detailed information of TA system TA025522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1257584..1258152
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MCAN_RS05940
• Protein ID: WP_003405865.1
• Coordinates: 1257778..1258152 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MCAN_RS05935
• Protein ID: WP_003405863.1
• Coordinates: 1257584..1257781 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS05915	1253625..1254263	-	639	WP_014000611.1	lipid droplet-associated protein	-
MCAN_RS05920	1254353..1255360	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MCAN_RS05925	1255377..1256360	-	984	WP_014000612.1	hypothetical protein	-
MCAN_RS05930	1256423..1257496	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MCAN_RS05935	1257584..1257781	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MCAN_RS05940	1257778..1258152	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS21185	1258355..1258592	+	238	Protein_1159	hypothetical protein	-
MCAN_RS05945	1258710..1258895	+	186	WP_003901093.1	hypothetical protein	-
MCAN_RS05950	1258822..1259097	-	276	WP_014000614.1	hypothetical protein	-
MCAN_RS05955	1259340..1259663	+	324	WP_014000615.1	antibiotic biosynthesis monooxygenase	-
MCAN_RS05960	1259678..1260388	-	711	WP_174887874.1	guanylate cyclase	-
MCAN_RS23050	1260410..1260619	+	210	WP_174887875.1	hypothetical protein	-
MCAN_RS05970	1260571..1261280	-	710	Protein_1165	adenylate/guanylate cyclase domain-containing protein	-
MCAN_RS05975	1261416..1262816	+	1401	WP_003898735.1	glucose-6-phosphate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T25522 WP_003405865.1 NC_015848:1257778-1258152 [Mycobacterium canettii CIPT 140010059]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T25522 NC_015848:1257778-1258152 [Mycobacterium canettii CIPT 140010059]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT25522 WP_003405863.1 NC_015848:1257584-1257781 [Mycobacterium canettii CIPT 140010059]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT25522 NC_015848:1257584-1257781 [Mycobacterium canettii CIPT 140010059]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07