Detailed information of TA system TA025521

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 1177267..1179041
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: -
• Locus tag: MCAN_RS05510
• Protein ID: WP_014000567.1
• Coordinates: 1177267..1178151 (-)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: G0TGF4
• Locus tag: MCAN_RS05515
• Protein ID: WP_014000568.1
• Coordinates: 1178154..1179041 (-)
• Length: 296 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS05485	1173364..1174191	-	828	WP_003405370.1	PE domain-containing protein	-
MCAN_RS05490	1174882..1175120	+	239	Protein_1077	hypothetical protein	-
MCAN_RS23270	1175339..1176375	+	1037	Protein_1078	IS3 family transposase	-
MCAN_RS05510	1177267..1178151	-	885	WP_014000567.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MCAN_RS05515	1178154..1179041	-	888	WP_014000568.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
MCAN_RS23105	1179291..1180507	+	1217	Protein_1081	site-specific integrase	-
MCAN_RS05535	1180647..1181765	-	1119	WP_014000570.1	site-specific integrase	-
MCAN_RS20785	1181752..1182282	-	531	WP_014000571.1	helix-turn-helix domain-containing protein	-
MCAN_RS05550	1182665..1183237	+	573	WP_014000572.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1175166..1190978	15812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-272)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-73)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 32917.43 Da
• Isoelectric Point: 6.6960

>T25521 WP_014000567.1 NC_015848:c1178151-1177267 [Mycobacterium canettii CIPT 140010059]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNAGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIAASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRTRFAFLTDLDADEQRWAACDERHRREVENALAALQS

Nucleotide

• Length: 885 bp

>T25521 NC_015848:c1178151-1177267 [Mycobacterium canettii CIPT 140010059]
GTGGCCGGACTGACCCGCGCGCTCGTCGCGCGTCACGCTCTTGGCCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGCCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGTGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCACTGGCAGCTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGCAGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCCTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCCGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
CAACCACTTCGCCTCGCGGACCATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGACGCGATTTGCGTTCCTCACCGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGCGCTGCAGT
CCTGA

Antitoxin

Protein

• Length: 296 a.a.
• Molecular weight: 32312.15 Da
• Isoelectric Point: 9.0338

>AT25521 WP_014000568.1 NC_015848:c1179041-1178154 [Mycobacterium canettii CIPT 140010059]
VGSPAGADRRIPTWASRVVSGLARDRPTVVTKQDLTLRLAEAGCERDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEDAV
SDPYLTLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPVKRLPDGLAPYVSVVRIPWNAADTALLAPRPAL
LVRRRLDIVAWATGLPAFGPEALLVQIATRPASFGPWADLVAHLDELIADCSDERLERLLSGRPTSAWQRASYLLDAAGE
PARGQALLAKRQTEAMPVARFTTAHSRDRGESVWAPEYQLVDELVAPLLRVIGKA

Nucleotide

• Length: 888 bp

>AT25521 NC_015848:c1179041-1178154 [Mycobacterium canettii CIPT 140010059]
GTGGGGAGCCCAGCCGGTGCCGATCGGCGCATCCCCACCTGGGCATCACGGGTGGTGTCCGGTCTCGCGCGCGATCGCCC
TACGGTCGTCACGAAGCAGGATCTGACGCTACGACTTGCAGAGGCCGGGTGTGAACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATCGGGTGGCTGGTCCAACTGCCGGTGAAAGGAACGTGGGCATTCATTCCCCCCGGCGAGGATGCAGTC
TCTGACCCGTATCTAACGCTGCGGTCGTGGCTGGCCCGCGACCAGAACGCAGGCTTCATGCTGGCTGGAGCATCGGCAGC
GTGGCACCTCGGTTACCTGGACCGCCAACCTGACGGCCGCATCCCGATCTGGCTACCGCCGGTCAAACGGCTCCCGGACG
GCCTAGCACCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCTGACACCGCACTGCTTGCTCCGCGTCCCGCGTTG
CTGGTACGGCGGCGGCTCGACATCGTCGCCTGGGCGACCGGGTTACCGGCATTCGGCCCGGAAGCACTACTCGTGCAAAT
CGCCACGCGCCCAGCCTCATTCGGGCCGTGGGCCGATCTAGTCGCCCACCTCGACGAACTCATCGCCGACTGCAGTGACG
AGCGCCTGGAGCGTCTGCTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACGCAGCCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGTCAAACAGAAGCGATGCCGGTGGCCCGATTCACCACCGCGCACAGCCG
CGACCGCGGCGAAAGCGTCTGGGCGCCGGAGTACCAGCTCGTCGACGAGCTCGTCGCACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Structures

Antitoxin

• AlphaFold DB: G0TGF4