Detailed information of TA system TA025520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1029448..1030421
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: G0TFR5
• Locus tag: MCAN_RS04810
• Protein ID: WP_014000498.1
• Coordinates: 1029921..1030421 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: G0TFR4
• Locus tag: MCAN_RS04805
• Protein ID: WP_003404754.1
• Coordinates: 1029448..1029924 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS04785	1025295..1026566	-	1272	WP_014000496.1	PPE family protein	-
MCAN_RS04790	1026581..1026880	-	300	WP_003404748.1	PE family protein	-
MCAN_RS04795	1027324..1029105	+	1782	WP_014000497.1	BCCT family transporter	-
MCAN_RS04800	1029102..1029359	+	258	WP_003404750.1	hypothetical protein	-
MCAN_RS04805	1029448..1029924	+	477	WP_003404754.1	DUF1778 domain-containing protein	Antitoxin
MCAN_RS04810	1029921..1030421	+	501	WP_014000498.1	GNAT family N-acetyltransferase	Toxin
MCAN_RS04820	1030734..1032053	-	1320	WP_014000499.1	IS256-like element IS1554 family transposase	-
MCAN_RS04825	1032343..1032924	+	582	WP_014000500.1	IS607-like element IS1535 family transposase	-
MCAN_RS04830	1032924..1034576	+	1653	WP_003404761.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18147.68 Da
• Isoelectric Point: 7.8659

>T25520 WP_014000498.1 NC_015848:1029921-1030421 [Mycobacterium canettii CIPT 140010059]
VSGYSAPRRISDADDVTRFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGWVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T25520 NC_015848:1029921-1030421 [Mycobacterium canettii CIPT 140010059]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGGTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTTGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTCGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17357.95 Da
• Isoelectric Point: 11.2467

>AT25520 WP_003404754.1 NC_015848:1029448-1029924 [Mycobacterium canettii CIPT 140010059]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT25520 NC_015848:1029448-1029924 [Mycobacterium canettii CIPT 140010059]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTGGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCCGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Structures

Toxin

• AlphaFold DB: G0TFR5

Antitoxin

• AlphaFold DB: G0TFR4