Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4435..5012 Replicon plasmid pMB9509_2
Accession NZ_CP103501
Organism Klebsiella pneumoniae strain 5589

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag M5S52_RS26875 Protein ID WP_059065587.1
Coordinates 4680..5012 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag M5S52_RS26870 Protein ID WP_032454911.1
Coordinates 4435..4680 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S52_RS26855 (M5S52_26855) 1..858 + 858 WP_259430743.1 incFII family plasmid replication initiator RepA -
M5S52_RS26860 (M5S52_26860) 2097..2732 + 636 WP_023317852.1 DUF2268 domain-containing putative Zn-dependent protease -
M5S52_RS26865 (M5S52_26865) 2868..3863 - 996 WP_020326536.1 IS110 family transposase -
M5S52_RS26870 (M5S52_26870) 4435..4680 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M5S52_RS26875 (M5S52_26875) 4680..5012 + 333 WP_059065587.1 endoribonuclease MazF Toxin
M5S52_RS26880 (M5S52_26880) 5272..5595 + 324 WP_021312689.1 hypothetical protein -
M5S52_RS26885 (M5S52_26885) 5778..6032 - 255 WP_009653916.1 hypothetical protein -
M5S52_RS26890 (M5S52_26890) 6108..6365 - 258 WP_004098928.1 hypothetical protein -
M5S52_RS26895 (M5S52_26895) 6414..6617 - 204 WP_004098931.1 HHA domain-containing protein -
M5S52_RS26900 (M5S52_26900) 6651..7019 - 369 WP_015344965.1 hypothetical protein -
M5S52_RS26905 (M5S52_26905) 7063..7557 - 495 WP_009310053.1 hypothetical protein -
M5S52_RS26910 (M5S52_26910) 7588..8154 - 567 WP_013023777.1 hypothetical protein -
M5S52_RS26915 (M5S52_26915) 8151..8414 - 264 WP_064175861.1 hypothetical protein -
M5S52_RS26920 (M5S52_26920) 8634..8840 + 207 Protein_13 hypothetical protein -
M5S52_RS26925 (M5S52_26925) 9001..9945 + 945 WP_020801688.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..76999 76999


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T255193 WP_059065587.1 NZ_CP103501:4680-5012 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT255193 WP_032454911.1 NZ_CP103501:4435-4680 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References