Detailed information of TA system TA025519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1019919..1020538
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MCAN_RS04755
• Protein ID: WP_003404726.1
• Coordinates: 1020104..1020538 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: MCAN_RS04750
• Protein ID: WP_003404724.1
• Coordinates: 1019919..1020098 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS04735	1015261..1016841	+	1581	WP_015287949.1	serine hydrolase	-
MCAN_RS04740	1016838..1019231	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
MCAN_RS21090	1019130..1019528	-	399	WP_080603695.1	transposase	-
MCAN_RS04750	1019919..1020098	+	180	WP_003404724.1	antitoxin	Antitoxin
MCAN_RS04755	1020104..1020538	+	435	WP_003404726.1	SRPBCC family protein	Toxin
MCAN_RS04760	1020636..1021409	+	774	WP_003404735.1	VOC family protein	-
MCAN_RS04765	1021474..1021923	+	450	WP_003404738.1	hypothetical protein	-
MCAN_RS04770	1022057..1022287	-	231	WP_003898642.1	hypothetical protein	-
MCAN_RS04775	1022454..1023962	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
MCAN_RS04780	1023964..1025202	-	1239	WP_014000495.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T25519 WP_003404726.1 NC_015848:1020104-1020538 [Mycobacterium canettii CIPT 140010059]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T25519 NC_015848:1020104-1020538 [Mycobacterium canettii CIPT 140010059]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT25519 WP_003404724.1 NC_015848:1019919-1020098 [Mycobacterium canettii CIPT 140010059]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT25519 NC_015848:1019919-1020098 [Mycobacterium canettii CIPT 140010059]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5