Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4672523..4673333 Replicon chromosome
Accession NZ_CP103499
Organism Klebsiella pneumoniae strain 5589

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M5S52_RS22850 Protein ID WP_004178461.1
Coordinates 4672523..4673056 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M5S52_RS22855 Protein ID WP_002887278.1
Coordinates 4673067..4673333 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S52_RS22845 (4671354) 4671354..4672475 + 1122 WP_002887282.1 cupin domain-containing protein -
M5S52_RS22850 (4672523) 4672523..4673056 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M5S52_RS22855 (4673067) 4673067..4673333 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M5S52_RS22860 (4673436) 4673436..4674869 - 1434 WP_009484674.1 Cu(+)/Ag(+) sensor histidine kinase -
M5S52_RS22865 (4674859) 4674859..4675542 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
M5S52_RS22870 (4675714) 4675714..4677099 + 1386 WP_009484676.1 efflux transporter outer membrane subunit -
M5S52_RS22875 (4677117) 4677117..4677461 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T255188 WP_004178461.1 NZ_CP103499:c4673056-4672523 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT255188 WP_002887278.1 NZ_CP103499:c4673333-4673067 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References