Detailed information of TA system TA025518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 851629..852338
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TEX5
• Locus tag: MCAN_RS03920
• Protein ID: WP_014000388.1
• Coordinates: 851910..852338 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TEX4
• Locus tag: MCAN_RS03915
• Protein ID: WP_003403834.1
• Coordinates: 851629..851886 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS22905	847246..847491	+	246	WP_031648253.1	hypothetical protein	-
MCAN_RS03890	847713..848036	+	324	Protein_772	transposase	-
MCAN_RS03895	848169..848696	+	528	WP_003901009.1	PE family protein	-
MCAN_RS03900	849074..849631	-	558	WP_003403820.1	hypothetical protein	-
MCAN_RS03905	849628..850134	-	507	WP_003403827.1	helix-turn-helix domain-containing protein	-
MCAN_RS03910	850252..851538	+	1287	Protein_776	PE family protein	-
MCAN_RS03915	851629..851886	+	258	WP_003403834.1	ribbon-helix-helix protein, CopG family	Antitoxin
MCAN_RS03920	851910..852338	+	429	WP_014000388.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS03925	852428..852602	-	175	Protein_779	transposase	-
MCAN_RS03930	852716..852961	+	246	WP_003403841.1	hypothetical protein	-
MCAN_RS03935	853030..853914	-	885	WP_003403844.1	3-hydroxyisobutyrate dehydrogenase	-
MCAN_RS03940	853925..855097	-	1173	WP_003403845.1	acyl-CoA dehydrogenase FadE9	-
MCAN_RS03945	855104..856636	-	1533	WP_003403847.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	847722..848036	314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-132)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15875.32 Da
• Isoelectric Point: 7.5536

>T25518 WP_014000388.1 NC_015848:851910-852338 [Mycobacterium canettii CIPT 140010059]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAAAFAFIDAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVEHHCAVVSLDRDFARFTSVRHIRPPL

Nucleotide

• Length: 429 bp

>T25518 NC_015848:851910-852338 [Mycobacterium canettii CIPT 140010059]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGCTGCATTCGCATTCATCGACGCCGTCACCGCCCAGCCCCACCACCTT
CCGACCAACCCCGGCCCCAGACACCTCATGCTGCTACGCAAACTCTGCGACGAGGCCGATGCGTCCGGCGACCTAATACC
CGACGCGGTACTCGCGGCCATAGCAGTGGAGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTCACCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9114.28 Da
• Isoelectric Point: 10.6530

>AT25518 WP_003403834.1 NC_015848:851629-851886 [Mycobacterium canettii CIPT 140010059]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT25518 NC_015848:851629-851886 [Mycobacterium canettii CIPT 140010059]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTCGG
CGCGGTGATCGAGGACGCCCTTCGCCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• AlphaFold DB: G0TEX5

Antitoxin

• AlphaFold DB: G0TEX4