Detailed information of TA system TA025517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 764713..765253
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TQD9
• Locus tag: MCAN_RS03420
• Protein ID: WP_014000341.1
• Coordinates: 764713..765021 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: MCAN_RS03425
• Protein ID: WP_003403381.1
• Coordinates: 765008..765253 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS03395	760028..761533	+	1506	WP_014000339.1	carotenoid cleavage oxygenase	-
MCAN_RS03400	761614..762624	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
MCAN_RS03405	763012..763395	-	384	WP_003403365.1	ribonuclease VapC6	-
MCAN_RS03410	763424..763645	-	222	WP_015292353.1	type II toxin-antitoxin system VapB family antitoxin	-
MCAN_RS03415	763721..764437	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
MCAN_RS03420	764713..765021	-	309	WP_014000341.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MCAN_RS03425	765008..765253	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
MCAN_RS03430	765363..765798	-	436	Protein_684	type II toxin-antitoxin system VapC family toxin	-
MCAN_RS03435	765795..766049	-	255	WP_162139305.1	antitoxin	-
MCAN_RS03440	766163..768526	+	2364	WP_014000343.1	arylsulfatase AtsD	-
MCAN_RS03445	768589..768915	+	327	WP_162139306.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MCAN_RS03450	768827..769240	+	414	WP_014000345.1	PIN domain-containing protein	-
MCAN_RS03455	769237..769410	+	174	WP_014000346.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11293.03 Da
• Isoelectric Point: 11.3260

>T25517 WP_014000341.1 NC_015848:c765021-764713 [Mycobacterium canettii CIPT 140010059]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAAEDRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T25517 NC_015848:c765021-764713 [Mycobacterium canettii CIPT 140010059]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
CCGAAGACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT25517 WP_003403381.1 NC_015848:c765253-765008 [Mycobacterium canettii CIPT 140010059]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT25517 NC_015848:c765253-765008 [Mycobacterium canettii CIPT 140010059]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: G0TQD9

Antitoxin

• AlphaFold DB: G0TQE0